Transcriptome profiling of the whitefly Bemisia tabaci reveals stage-specific gene expression signatures for thiamethoxam resistance.
Insect Mol Biol, 2013/10;22(5):485-96.
Yang N[1], Xie W, Jones CM, Bass C, Jiao X, Yang X, Liu B, Li R, Zhang Y
Affiliations
PMID: 23889345DOI: 10.1111/imb.12038
Impact factor: 3.424
Abstract
Bemisia tabaci has developed high levels of resistance to many insecticides including the neonicotinoids and there is strong evidence that for some compounds resistance is stage-specific. To investigate the molecular basis of B. tabaci resistance to the neonicotinoid thiamethoxam we used a custom whitefly microarray to compare gene expression in the egg, nymph and adult stages of a thiamethoxam-resistant strain (TH-R) with a susceptible strain (TH-S). Gene ontology and bioinformatic analyses revealed that in all life stages many of the differentially expressed transcripts encoded enzymes involved in metabolic processes and/or metabolism of xenobiotics. Several of these are candidate resistance genes and include the cytochrome P450 CYP6CM1, which has been shown to confer resistance to several neonicotinoids previously, a P450 belonging to the Cytochrome P450s 4 family and a glutathione S-transferase (GST) belonging to the sigma class. Finally several ATP-binding cassette transporters of the ABCG subfamily were highly over-expressed in the adult stage of the TH-R strain and may play a role in resistance by active efflux. Here, we evaluated both common and stage-specific gene expression signatures and identified several candidate resistance genes that may underlie B. tabaci resistance to thiamethoxam.
Keywords: Bemisia tabaci; metabolic resistance; microarray; stage specific; thiamethoxam
MeSH terms
Animals; Female; Gene Expression Profiling; Hemiptera; Insecticide Resistance; Life Cycle Stages; Neonicotinoids; Nitro Compounds; Oligonucleotide Array Sequence Analysis; Oxazines; Reverse Transcriptase Polymerase Chain Reaction; Thiamethoxam; Thiazoles; Transcriptome
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