High-resolution analysis of cis-acting regulatory networks at the α-globin locus.
Philos Trans R Soc Lond B Biol Sci, 2013;368(1620):20120361.
Hughes JR[1], Lower KM, Dunham I, Taylor S, De Gobbi M, Sloane-Stanley JA, McGowan S, Ragoussis J, Vernimmen D, Gibbons RJ, Higgs DR
Affiliations
PMID: 23650635DOI: 10.1098/rstb.2012.0361
Impact factor: 6.671
Abstract
We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the α-globin cluster.
Keywords: chromatin conformation capture; cis-regulatory elements; α-globin locus
MeSH terms
CCCTC-Binding Factor; Chromatin Assembly and Disassembly; Chromosomes, Human, Pair 16; Gene Regulatory Networks; Genetic Loci; Genome, Human; Humans; Open Reading Frames; Promoter Regions, Genetic; Protein Interaction Mapping; Regulatory Sequences, Nucleic Acid; Repressor Proteins; alpha-Globins; beta-Globins
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