Clostridium botulinum strain Af84 contains three neurotoxin gene clusters: bont/A2, bont/F4 and bont/F5.
PLoS One, 2013;8(4):e61205.
Dover N[1], Barash JR, Hill KK, Davenport KW, Teshima H, Xie G, Arnon SS
Affiliations
PMID: 23637798DOI: 10.1371/journal.pone.0061205
Impact factor: 3.752
Abstract
Sanger and shotgun sequencing of Clostridium botulinum strain Af84 type Af and its botulinum neurotoxin gene (bont) clusters identified the presence of three bont gene clusters rather than the expected two. The three toxin gene clusters consisted of bont subtypes A2, F4 and F5. The bont/A2 and bont/F4 gene clusters were located within the chromosome (the latter in a novel location), while the bont/F5 toxin gene cluster was located within a large 246 kb plasmid. These findings are the first identification of a C. botulinum strain that contains three botulinum neurotoxin gene clusters.
MeSH terms
Animals; Botulinum Toxins; Botulinum Toxins, Type A; Chromosomes, Bacterial; Clostridium botulinum; Genomics; Mice; Multigene Family; Neurotoxins; Plasmids; Sequence Analysis
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