Comparison of endogenous and overexpressed MyoD shows enhanced binding of physiologically bound sites.
Skelet Muscle, 2013/4/08;3(1):8.
Yao Z[1], Fong AP[2, 3], Cao Y[4], Ruzzo WL[5], Gentleman RC[4], Tapscott SJ[1, 2, 6]
Affiliations
PMID: 23566431DOI: 10.1186/2044-5040-3-8
Impact factor: 5.063
Abstract
background: Transcription factor overexpression is common in biological experiments and transcription factor amplification is associated with many cancers, yet few studies have directly compared the DNA-binding profiles of endogenous versus overexpressed transcription factors.
methods: We analyzed MyoD ChIP-seq data from C2C12 mouse myotubes, primary mouse myotubes, and mouse fibroblasts differentiated into muscle cells by overexpression of MyoD and compared the genome-wide binding profiles and binding site characteristics of endogenous and overexpressed MyoD.
results: Overexpressed MyoD bound to the same sites occupied by endogenous MyoD and possessed the same E-box sequence preference and co-factor site enrichments, and did not bind to new sites with distinct characteristics.
conclusions: Our data demonstrate a robust fidelity of transcription factor binding sites over a range of expression levels and that increased amounts of transcription factor increase the binding at physiologically bound sites.
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download