HCFC1 is a common component of active human CpG-island promoters and coincides with ZNF143, THAP11, YY1, and GABP transcription factor occupancy.
Genome Res, 2013/6;23(6):907-16.
Michaud J[1], Praz V, James Faresse N, Jnbaptiste CK, Tyagi S, Schütz F, Herr W
Affiliations
PMID: 23539139DOI: 10.1101/gr.150078.112
Impact factor: 9.438
Abstract
In human transcriptional regulation, DNA-sequence-specific factors can associate with intermediaries that orchestrate interactions with a diverse set of chromatin-modifying enzymes. One such intermediary is HCFC1 (also known as HCF-1). HCFC1, first identified in herpes simplex virus transcription, has a poorly defined role in cellular transcriptional regulation. We show here that, in HeLa cells, HCFC1 is observed bound to 5400 generally active CpG-island promoters. Examination of the DNA sequences underlying the HCFC1-binding sites revealed three sequence motifs associated with the binding of (1) ZNF143 and THAP11 (also known as Ronin), (2) GABP, and (3) YY1 sequence-specific transcription factors. Subsequent analysis revealed colocalization of HCFC1 with these four transcription factors at ∼90% of the 5400 HCFC1-bound promoters. These studies suggest that a relatively small number of transcription factors play a major role in HeLa-cell transcriptional regulation in association with HCFC1.
MeSH terms
Base Sequence; Binding Sites; CpG Islands; GA-Binding Protein Transcription Factor; Gene Expression Regulation; HeLa Cells; Host Cell Factor C1; Humans; Nucleotide Motifs; Position-Specific Scoring Matrices; Promoter Regions, Genetic; Protein Binding; RNA, Messenger; Repressor Proteins; Signal Transduction; Trans-Activators; Transcription Initiation Site; Transcriptional Activation; YY1 Transcription Factor
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