Genetic characterization of pPHDP60, a novel conjugative plasmid from the marine fish pathogen Photobacterium damselae subsp. piscicida.
Plasmid, 2013/7;70(1):154-9.
Balado M[1], Lemos ML, Osorio CR
Affiliations
PMID: 23474463
Impact factor: 3.085
Abstract
A new plasmid designated pPHDP60 from a strain of the marine bacterium Photobacterium damselae subsp. piscicida isolated from diseased seabream has been characterised. pPHDP60 consists of 59,731bp, has a G+C content of 37.2% and encodes 63 predicted open-reading frames (ORFs). The plasmid backbone sequence includes, among other genes, 15 ORFs homologous to proteins of type IV conjugation systems described in IncP-type plasmids. Two modules could be distinguished within pPHDP60 sequence. One module included 10 genes of a putative type II secretion system with homologues in other Photobacterium and Vibrio plasmids. A second module exhibiting a transposon structure included a functional haloalkane dehalogenase gene linB as well as a toxin/antitoxin system. Additional interesting features of pPHDP60 include its ability to be conjugally transferred to several Gram negative bacteria.
MeSH terms
Animals; Bacterial Proteins; Base Composition; Base Sequence; Conjugation, Genetic; DNA Replication; DNA Transposable Elements; DNA, Bacterial; Molecular Sequence Data; Open Reading Frames; Operon; Photobacterium; Plasmids; Sea Bream; Sequence Analysis, DNA
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