Complete genome sequence of Liberibacter crescens BT-1.

Stand Genomic Sci, 2012/12/19;7(2):271-83.

Leonard MT[1], Fagen JR, Davis-Richardson AG, Davis MJ, Triplett EW

Affiliations

PMID: 23408754DOI: 10.4056/sigs.3326772

Abstract
Liberibacter crescens BT-1, a Gram-negative, rod-shaped bacterial isolate, was previously recovered from mountain papaya to gain insight on Huanglongbing (HLB) and Zebra Chip (ZC) diseases. The genome of BT-1 was sequenced at the Interdisciplinary Center for Biotechnology Research (ICBR) at the University of Florida. A finished assembly and annotation yielded one chromosome with a length of 1,504,659 bp and a G+C content of 35.4%. Comparison to other species in the Liberibacter genus, L. crescens has many more genes in thiamine and essential amino acid biosynthesis. This likely explains why L. crescens BT-1 is culturable while the known Liberibacter strains have not yet been cultured. Similar to CandidatusL. asiaticus psy62, the L. crescens BT-1 genome contains two prophage regions.

Keywords: Huanglongbing; citrus greening; fastidious; hybrid assembly; reduced genome

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