A new structural paradigm in copper resistance in Streptococcus pneumoniae.
Nat Chem Biol, 2013/3;9(3):177-83.
Fu Y[1], Tsui HC, Bruce KE, Sham LT, Higgins KA, Lisher JP, Kazmierczak KM, Maroney MJ, Dann CE 3rd, Winkler ME, Giedroc DP
Affiliations
PMID: 23354287DOI: 10.1038/nchembio.1168
Impact factor: 16.174
Abstract
Copper resistance has emerged as an important virulence determinant of microbial pathogens. In Streptococcus pneumoniae, copper resistance is mediated by the copper-responsive repressor CopY, CupA and the copper-effluxing P(1B)-type ATPase CopA. We show here that CupA is a previously uncharacterized cell membrane-anchored Cu(I) chaperone and that a Cu(I) binding-competent, membrane-localized CupA is obligatory for copper resistance. The crystal structures of the soluble domain of CupA and the N-terminal metal-binding domain (MBD) of CopA (CopA(MBD)) reveal isostructural cupredoxin-like folds that each harbor a binuclear Cu(I) cluster unprecedented in bacterial copper trafficking. NMR studies reveal unidirectional Cu(I) transfer from the low-affinity site on the soluble domain of CupA to the high-affinity site of CopA(MBD). However, copper binding by CopA(MBD) is not essential for cellular copper resistance, consistent with a primary role of CupA in cytoplasmic Cu(I) sequestration and/or direct delivery to the transmembrane site of CopA for cellular efflux.
MeSH terms
Bacterial Proteins; Binding Sites; Cell Membrane; Copper; Crystallography, X-Ray; Drug Resistance, Bacterial; Magnetic Resonance Spectroscopy; Models, Molecular; Mutation; Protein Structure, Tertiary; Streptococcus pneumoniae
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