Genome-wide quantitative enhancer activity maps identified by STARR-seq.
Science, 2013/3/01;339(6123):1074-7.
Arnold CD[1], Gerlach D, Stelzer C, Boryń ŁM, Rath M, Stark A
Affiliations
PMID: 23328393DOI: 10.1126/science.1232542
Impact factor: 63.714
Abstract
Genomic enhancers are important regulators of gene expression, but their identification is a challenge, and methods depend on indirect measures of activity. We developed a method termed STARR-seq to directly and quantitatively assess enhancer activity for millions of candidates from arbitrary sources of DNA, which enables screens across entire genomes. When applied to the Drosophila genome, STARR-seq identifies thousands of cell type-specific enhancers across a broad continuum of strengths, links differential gene expression to differences in enhancer activity, and creates a genome-wide quantitative enhancer map. This map reveals the highly complex regulation of transcription, with several independent enhancers for both developmental regulators and ubiquitously expressed genes. STARR-seq can be used to identify and quantify enhancer activity in other eukaryotes, including humans.
MeSH terms
Animals; Chromosome Mapping; Drosophila melanogaster; Enhancer Elements, Genetic; Female; Gene Expression Regulation; Gene Expression Regulation, Developmental; Genome; HeLa Cells; Humans; Ovary; Sequence Analysis, DNA; Transcription, Genetic
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