Feeder-independent derivation of induced-pluripotent stem cells from peripheral blood endothelial progenitor cells.
Stem Cell Res, 2013/3;10(2):195-202.
Chang WY[1], Lavoie JR, Kwon SY, Chen Z, Manias JL, Behbahani J, Ling V, Kandel RA, Stewart DJ, Stanford WL
Affiliations
PMID: 23291290
Impact factor: 1.587
Abstract
Induced-pluripotent stem cells (iPSCs) are a potential alternative cell source in regenerative medicine, which includes the use of differentiated iPSCs for cell therapies to treat coronary artery and/or peripheral arterial diseases. Late-outgrowth endothelial progenitor cells (late-EPCs) are a unique primary cell present in peripheral blood that exhibit high proliferative capacity, are being used in a wide variety of clinical trials, and have the ability to differentiate into mature endothelial cells. The objective of this study was to reprogram peripheral blood-derived late-EPCs to a pluripotent state under feeder-free and defined culture conditions. Late-EPCs that were retrovirally transduced with OCT4, SOX2, KLF4, c-MYC, and iPSC colonies were derived in feeder-free and defined media conditions. EPC-iPSCs expressed pluripotent markers, were capable of differentiating to cells from all three germ-layers, and retained a normal karyotype. Transcriptome analyses demonstrated that EPC-iPSCs exhibit a global gene expression profile similar to human embryonic stem cells (hESCs). We have generated iPSCs from late-EPCs under feeder-free conditions. Thus, peripheral blood-derived late-outgrowth EPCs represent an alternative cell source for generating iPSCs.
MeSH terms
Biomarkers; Cell Differentiation; Cell Proliferation; Cellular Reprogramming; Embryonic Stem Cells; Endothelial Cells; Feeder Cells; Humans; Induced Pluripotent Stem Cells; Karyotyping; Kruppel-Like Factor 4; Retroviridae; Transcriptome
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