The organization of naphthalene degradation genes in Pseudomonas putida strain AK5.
Res Microbiol, 2013/4;164(3):244-53.
Izmalkova TY[1], Sazonova OI, Nagornih MO, Sokolov SL, Kosheleva IA, Boronin AM
Affiliations
PMID: 23266498
Impact factor: 3.946
Abstract
The Pseudomonas putida АК5 that was isolated from the slime pit of a Nizhnekamsk oil chemical factory can metabolize naphthalene via salicylate and gentisate. Catabolic genes are localized on non-conjugative IncP-7 plasmid pAK5 of about 115 kb in size. The "classical"nah-1 operon and the novel sgp-operon (salicylate-gentisate pathway) are both involved in naphthalene degradation by P. putida АК5, that was first described for Pseudomonas. The sgp-operon includes six open reading frames (ORFs) (sgpAIKGHB). The four ORFs code for the entire salicylate 5-hydroxylase - oxidoreductase component (sgpA), large and small subunits of the oxigenase component (sgpG and sgpH) and 2Fe-2S ferredoxin (sgpB). Genes for gentisate 1, 2-dioxygenase (sgpI) and fumarylpyruvate hydrolase (sgpK) are located in salicylate 5-hydroxylase genes clustering between sgpA and sgpG. The putative positive regulator for the sgp-operon (sgpR) was found upstream of the sgpA gene and oriented in the opposite direction from sgpA. The putative maleylacetoacetate isomerase gene is located apart, directly downstream from the sgp-operon. The sgp-operon organization and phylogenetic analysis of deduced amino acid sequences indicate that this operon has a mosaic structure according to the modular theory of the evolution of modern catabolic pathways.
MeSH terms
Amino Acid Sequence; Bacterial Proteins; DNA, Bacterial; Dioxygenases; Gentisates; Hydrolases; Mixed Function Oxygenases; Molecular Sequence Data; Naphthalenes; Open Reading Frames; Operon; Oxidoreductases; Phylogeny; Pseudomonas putida; Salicylates; cis-trans-Isomerases
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