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23221834
Early cone setting in Picea abies acrocona is associated with increased transcriptional activity of a MADS box transcription factor.

Plant Physiol, 2013/2;161(2):813-23.

Uddenberg D[1], Reimegård J, Clapham D, Almqvist C, von Arnold S, Emanuelsson O, Sundström JF

Affiliations

PMID: 23221834DOI: 10.1104/pp.112.207746

Impact factor: 8.005

Abstract
Conifers normally go through a long juvenile period, for Norway spruce (Picea abies) around 20 to 25 years, before developing male and female cones. We have grown plants from inbred crosses of a naturally occurring spruce mutant (acrocona). One-fourth of the segregating acrocona plants initiate cones already in their second growth cycle, suggesting control by a single locus. The early cone-setting properties of the acrocona mutant were utilized to identify candidate genes involved in vegetative-to-reproductive phase change in Norway spruce. Poly(A(+)) RNA samples from apical and basal shoots of cone-setting and non-cone-setting plants were subjected to high-throughput sequencing (RNA-seq). We assembled and investigated 33,383 expressed putative protein-coding acrocona transcripts. Eight transcripts were differentially expressed between selected sample pairs. One of these (Acr42124_1) was significantly up-regulated in apical shoot samples from cone-setting acrocona plants, and the encoded protein belongs to the MADS box gene family of transcription factors. Using quantitative real-time polymerase chain reaction with independently derived plant material, we confirmed that the MADS box gene is up-regulated in both needles and buds of cone-inducing shoots when reproductive identity is determined. Our results constitute important steps for the development of a rapid cycling model system that can be used to study gene function in conifers. In addition, our data suggest the involvement of a MADS box transcription factor in the vegetative-to-reproductive phase change in Norway spruce.
MeSH terms
Crosses, Genetic; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; High-Throughput Nucleotide Sequencing; MADS Domain Proteins; Mutation; Norway; Phenotype; Phylogeny; Picea; Plant Leaves; Plant Proteins; Plant Shoots; Reverse Transcriptase Polymerase Chain Reaction; Sweden
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