Yamadazyma siamensis sp. nov., Yamadazyma phyllophila sp. nov. and Yamadazyma paraphyllophila sp. nov., three novel yeast species isolated from phylloplane in Thailand and Taiwan.
Antonie Van Leeuwenhoek, 2013/4;103(4):777-88.
Kaewwichian R[1], Yongmanitchai W, Kawasaki H, Wang PH, Yang SH, Limtong S
Affiliations
PMID: 23208204DOI: 10.1007/s10482-012-9860-6
Impact factor: 2.158
Abstract
Four strains representing three novel anamorphic yeast species were isolated from the external surface of sugarcane leaves (DMKU-RK254(T)), corn leaves (DMKU-RK548(T)), bean leaves (K129) in Thailand and hengchun pencilwood leaves (TrB1-1(T)) in Taiwan. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene, the internal transcribed spacer (ITS) region, the actin gene (ACT1) and the elongation factor 2 gene (EF2), the four strains were determined to represent novel Yamadazyma species although formation of ascospores was not observed. Strain DMKU-RK254(T) was determined to be related to Candida diddensiae, Candida naeodendra and Candida kanchanaburiensis but with 1.8, 1.8 and 2.0 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, respectively. It was assigned to Yamadazyma siamensis sp. nov. (type strain DMKU-RK254(T) = BCC 50730(T) = NBRC 108901(T) = CBS 12573(T)). The sequences of the D1/D2 region of the LSU rRNA gene, the ITS region, ACT1 gene and EF2 gene of two strains (DMKU-RK548(T) and K129) were identical but differed from that of strain TrB1-1(T) by 0.6, 1.0, 3.3 and 5.9 % nucleotide substitutions, respectively. Therefore, the two strains (DMKU-RK548(T) and K129) and strain TrB1-1(T) were assigned to be two separate species. The closest species in terms of pairwise sequences similarity of the D1/D2 region to the two novel species was Yamadazyma philogaea but with 1.1-1.7 % nucleotide substitutions. The two strains (DMKU-RK548(T) and K129) were assigned to Yamadazyma phyllophila sp. nov. (type strain DMKU-RK548(T) = BCC 50736(T) = NBRC 108906(T) = CBS 12572(T)) and the strain TrB1-1(T) was named Yamadazyma paraphyllophila sp. nov. (type strain TrB1-1(T) = BCRC 23030(T) = CCTCC AY 204005(T) = CBS 9928(T)).
MeSH terms
Cluster Analysis; DNA, Fungal; DNA, Ribosomal; Fabaceae; Genes, rRNA; Molecular Sequence Data; Mycological Typing Techniques; Phylogeny; Plant Leaves; RNA, Fungal; RNA, Ribosomal; Saccharomycetales; Saccharum; Sequence Analysis, DNA; Taiwan; Thailand; Zea mays
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