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23143274
Transcriptional repression of the APC/C activator CCS52A1 promotes active termination of cell growth.

EMBO J, 2012/12/12;31(24):4488-501.

Breuer C[1], Morohashi K, Kawamura A, Takahashi N, Ishida T, Umeda M, Grotewold E, Sugimoto K

Affiliations

PMID: 23143274DOI: 10.1038/emboj.2012.294

Impact factor: 14.012

Abstract
Spatial and temporal control of cell growth is central for the morphogenesis of multicellular organisms. For some cell types that undergo extensive post-mitotic cell growth, such as neurons and hair cells, orchestrating the extent of post-mitotic cell growth with development is vital for their physiology and function. Previous studies suggested that the extent of cell growth is linked with an increase in ploidy by endoreduplication but how developmental signals control endocycling and cell growth is not understood in both animals and plants. In this study we show that a trihelix transcription factor, GT2-LIKE 1 (GTL1), actively terminates ploidy-dependent cell growth and its developmentally regulated expression is one of the key determinants of cell size in Arabidopsis leaf hair cells (trichomes). Through genome-wide chromatin-binding studies (ChIP-chip) coupled with transcriptional profiling, we further demonstrate that GTL1 directly represses the transcription of CDH1/FZR/CCS52, an activator of the anaphase-promoting complex/cyclosome (APC/C), to stop the endocycle progression and ploidy-dependent cell growth. Thus, our findings uncover a previously uncharacterised key molecular link between developmental programming and cell-size control, highlighting the central role of APC/C in post-mitotic cell growth.
MeSH terms
Anaphase-Promoting Complex-Cyclosome; Arabidopsis; Arabidopsis Proteins; Base Sequence; Cell Cycle Proteins; Cell Growth Processes; Cell Size; Chromatin Immunoprecipitation; Gene Expression Profiling; Gene Expression Regulation, Plant; Microarray Analysis; Microscopy, Confocal; Molecular Sequence Data; Oligonucleotides; Plant Leaves; Plasmids; Ploidies; Real-Time Polymerase Chain Reaction; Transcription Factors; Two-Hybrid System Techniques; Ubiquitin-Protein Ligase Complexes
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