A physical, genetic and functional sequence assembly of the barley genome.
Nature, 2012/11/29;491(7426):711-6.
International Barley Genome Sequencing Consortium, Mayer KF, Waugh R, Brown JW, Schulman A, Langridge P, Platzer M, Fincher GB, Muehlbauer GJ, Sato K, Close TJ, Wise RP, Stein N
PMID: 23075845DOI: 10.1038/nature11543
Impact factor: 69.504
Abstract
Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
MeSH terms
Alternative Splicing; Codon, Nonsense; Crops, Agricultural; Evolution, Molecular; Gene Expression Regulation, Plant; Genes, Plant; Genome, Plant; Genomics; Hordeum; Molecular Sequence Annotation; Physical Chromosome Mapping; Polymorphism, Single Nucleotide; Repetitive Sequences, Nucleic Acid; Sequence Analysis, DNA; Transcriptome
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