Mouse ooplasm confers context-specific reprogramming capacity.
Nat Genet, 2012/9;44(9):978-80.
Chan MM[1], Smith ZD, Egli D, Regev A, Meissner A
Affiliations
PMID: 22902786DOI: 10.1038/ng.2382
Impact factor: 41.307
Abstract
Enucleated oocytes have the distinctive ability to reprogram somatic nuclei back to totipotency. Here, we investigate genome-scale DNA methylation patterns after nuclear transfer and compare them to the dynamics at fertilization. We identify specific targets for DNA demethylation after nuclear transfer, such as germline-associated promoters, as well as unique limitations that include certain repetitive element classes.
MeSH terms
Animals; Cytoplasm; DNA Methylation; Embryo, Mammalian; Embryonic Development; Female; Male; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Nuclear Transfer Techniques; Oocytes; Organ Specificity; Promoter Regions, Genetic; Sperm-Ovum Interactions
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