The BEAF-32 insulator coordinates genome organization and function during the evolution of Drosophila species.
Genome Res, 2012/11;22(11):2199-207.
Affiliations
PMID: 22895281DOI: 10.1101/gr.142125.112
Impact factor: 9.438
Abstract
Understanding the relationship between genome organization and expression is central to understanding genome function. Closely apposed genes in a head-to-head orientation share the same upstream region and are likely to be coregulated. Here we identify the Drosophila BEAF-32 insulator as a cis regulatory element separating close head-to-head genes with different transcription regulation modes. We then compare the binding landscapes of the BEAF-32 insulator protein in four different Drosophila genomes and highlight the evolutionarily conserved presence of this protein between close adjacent genes. We find that changes in binding of BEAF-32 to sites in the genome of different Drosophila species correlate with alterations in genome organization caused by DNA rearrangements or genome size expansion. The cross-talk between BEAF-32 genomic distribution and genome organization contributes to new gene-expression profiles, which in turn translate into specific and distinct phenotypes. The results suggest a mechanism for the establishment of differences in transcription patterns during evolution.
MeSH terms
Animals; Binding Sites; DNA-Binding Proteins; Drosophila; Drosophila Proteins; Evolution, Molecular; Eye Proteins; Gene Rearrangement; Genome, Insect; Insulator Elements; Phenotype; Species Specificity; Transcription, Genetic
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