Constitutive expression of clathrin hub hinders elicitor-induced clathrin-mediated endocytosis and defense gene expression in plant cells.
FEBS Lett, 2012/9/21;586(19):3293-8.
Adam T[1], Bouhidel K, Der C, Robert F, Najid A, Simon-Plas F, Leborgne-Castel N
Affiliations
PMID: 22796492
Impact factor: 3.864
Abstract
Endocytosis has been recently implicated in the signaling network associated with the recognition of microbes by plants. In a previous study, we showed that the elicitor cryptogein was able to induce clathrin-mediated endocytosis (CME) in tobacco suspension cells. Herein, we investigate further the induced CME by means of a GFP-tagged clathrin light chain and a CME inhibitor, the hub domain of clathrin heavy chain. Hub constitutive expression does affect neither cell growth nor constitutive endocytosis but abolishes cryptogein-induced CME. Such an inhibition has no impact on early events in the cryptogein signaling pathway but reduces the expression of defense-associated genes.
MeSH terms
Cell Line; Cell Membrane; Clathrin; Clathrin Heavy Chains; Clathrin Light Chains; Endocytosis; Fungal Proteins; Gene Expression; Genes, Plant; Microscopy, Electron, Transmission; Plant Proteins; Plants, Genetically Modified; RNA, Messenger; RNA, Plant; Recombinant Fusion Proteins; Signal Transduction; Nicotiana
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