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22763442
IDH1(R132H) mutation increases murine haematopoietic progenitors and alters epigenetics.

Nature, 2012/8/30;488(7413):656-9.

Sasaki M[1], Knobbe CB, Munger JC, Lind EF, Brenner D, Brüstle A, Harris IS, Holmes R, Wakeham A, Haight J, You-Ten A, Li WY, Schalm S, Su SM, Virtanen C, Reifenberger G, Ohashi PS, Barber DL, Figueroa ME, Melnick A, Zúñiga-Pflücker JC, Mak TW

Affiliations

PMID: 22763442DOI: 10.1038/nature11323

Impact factor: 69.504

Abstract
Mutations in the IDH1 and IDH2 genes encoding isocitrate dehydrogenases are frequently found in human glioblastomas and cytogenetically normal acute myeloid leukaemias (AML). These alterations are gain-of-function mutations in that they drive the synthesis of the ‘oncometabolite’ R-2-hydroxyglutarate (2HG). It remains unclear how IDH1 and IDH2 mutations modify myeloid cell development and promote leukaemogenesis. Here we report the characterization of conditional knock-in (KI) mice in which the most common IDH1 mutation, IDH1(R132H), is inserted into the endogenous murine Idh1 locus and is expressed in all haematopoietic cells (Vav-KI mice) or specifically in cells of the myeloid lineage (LysM-KI mice). These mutants show increased numbers of early haematopoietic progenitors and develop splenomegaly and anaemia with extramedullary haematopoiesis, suggesting a dysfunctional bone marrow niche. Furthermore, LysM-KI cells have hypermethylated histones and changes to DNA methylation similar to those observed in human IDH1- or IDH2-mutant AML. To our knowledge, our study is the first to describe the generation and characterization of conditional IDH1(R132H)-KI mice, and also the first report to demonstrate the induction of a leukaemic DNA methylation signature in a mouse model. Our report thus sheds light on the mechanistic links between IDH1 mutation and human AML.
MeSH terms
Aging; Animals; Bone Marrow; Cell Lineage; CpG Islands; DNA Methylation; Disease Models, Animal; Epigenesis, Genetic; Female; Gene Knock-In Techniques; Glioma; Hematopoiesis; Hematopoietic Stem Cells; Histones; Humans; Isocitrate Dehydrogenase; Leukemia, Myeloid, Acute; Male; Mice; Mutant Proteins; Mutation; Myeloid Cells; Spleen
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