Proteomics-based dissection of human endoderm progenitors by differential cell capture on antibody array.
Mol Cell Proteomics, 2012/9;11(9):586-95.
Sharivkin R[1], Walker MD, Soen Y
Affiliations
PMID: 22580589DOI: 10.1074/mcp.M111.016840
Impact factor: 7.381
Abstract
Heterogeneity, shortage of material, and lack of progenitor-specific cell surface markers are major obstacles to elucidating the mechanisms underlying developmental processes. Here we report a proteomics platform that alleviates these difficulties and demonstrate its effectiveness in fractionating heterogeneous cultures of early endoderm derived from human embryonic stem cells. The approach, designated differential cell-capture antibody array, is based on highly parallel, comparative screening of live cell populations using hundreds of antibodies directed against cell-surface antigens. We used this platform to fractionate the hitherto unresolved early endoderm compartment of CXCR4+ cells and identify several endoderm (CD61+ and CD63+) and non-endoderm (CD271+, CD49F+, CD44+ and B2M+) sub-populations. We provide evidence that one of these sub-populations, CD61+, is directly derived from CXCR4+ cells, displays characteristic kinetics of emergence, and exhibits a distinct gene expression profile. The results demonstrate the potential of the cell-capture antibody array as a powerful proteomics tool for detailed dissection of heterogeneous cellular systems.
MeSH terms
Antibodies; Antigens, Surface; Biomarkers; Cell Differentiation; Cell Line; Cell Lineage; Cell Separation; Embryonic Stem Cells; Endoderm; Flow Cytometry; Humans; Hyaluronan Receptors; Integrin alpha6; Integrin beta3; Nerve Tissue Proteins; Oligonucleotide Array Sequence Analysis; Proteomics; Receptors, CXCR4; Receptors, Nerve Growth Factor; Tetraspanin 30
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download