Recurrent variations in DNA methylation in human pluripotent stem cells and their differentiated derivatives.
Cell Stem Cell, 2012/5/04;10(5):620-34.
Nazor KL[1], Altun G, Lynch C, Tran H, Harness JV, Slavin I, Garitaonandia I, Müller FJ, Wang YC, Boscolo FS, Fakunle E, Dumevska B, Lee S, Park HS, Olee T, D'Lima DD, Semechkin R, Parast MM, Galat V, Laslett AL, Schmidt U, Keirstead HS, Loring JF, Laurent LC
Affiliations
PMID: 22560082DOI: 10.1016/j.stem.2012.02.013
Impact factor: 25.269
Abstract
Human pluripotent stem cells (hPSCs) are potential sources of cells for modeling disease and development, drug discovery, and regenerative medicine. However, it is important to identify factors that may impact the utility of hPSCs for these applications. In an unbiased analysis of 205 hPSC and 130 somatic samples, we identified hPSC-specific epigenetic and transcriptional aberrations in genes subject to X chromosome inactivation (XCI) and genomic imprinting, which were not corrected during directed differentiation. We also found that specific tissue types were distinguished by unique patterns of DNA hypomethylation, which were recapitulated by DNA demethylation during in vitro directed differentiation. Our results suggest that verification of baseline epigenetic status is critical for hPSC-based disease models in which the observed phenotype depends on proper XCI or imprinting and that tissue-specific DNA methylation patterns can be accurately modeled during directed differentiation of hPSCs, even in the presence of variations in XCI or imprinting.
MeSH terms
Cell Differentiation; Cells, Cultured; Chromosome Aberrations; Chromosomes, Human, X; DNA Methylation; Genetic Variation; Genomic Imprinting; Humans; Organ Specificity; Pluripotent Stem Cells; Recurrence; Stem Cell Niche; X Chromosome Inactivation
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