Interaction between NANOS2 and the CCR4-NOT deadenylation complex is essential for male germ cell development in mouse.
PLoS One, 2012;7(3):e33558.
Suzuki A[1], Saba R, Miyoshi K, Morita Y, Saga Y
Affiliations
PMID: 22448252DOI: 10.1371/journal.pone.0033558
Impact factor: 3.752
Abstract
Nanos is one of the evolutionarily conserved proteins implicated in germ cell development and we have previously shown that it interacts with the CCR4-NOT deadenylation complex leading to the suppression of specific RNAs. However, the molecular mechanism and physiological significance of this interaction have remained elusive. In our present study, we identify CNOT1, a component of the CCR4-NOT deadenylation complex, as a direct factor mediating the interaction with NANOS2. We find that the first 10 amino acids (AAs) of NANOS2 are required for this binding. We further observe that a NANOS2 mutant lacking these first 10 AAs (NANOS2-ΔN10) fails to rescue defects in the Nanos2-null mouse. Our current data thus indicate that the interaction with the CCR4-NOT deadenylation complex is essential for NANOS2 function. In addition, we further demonstrate that NANOS2-ΔN10 can associate with specific mRNAs as well as wild-type NANOS2, suggesting the existence of other NANOS2-associated factor(s) that determine the specificity of RNA-binding independently of the CCR4-NOT deadenylation complex.
MeSH terms
Animals; Biomarkers, Tumor; Blotting, Western; Carrier Proteins; Embryo, Mammalian; Gene Expression Profiling; HeLa Cells; Humans; Immunoprecipitation; Male; Mice; Mice, Knockout; Mice, Transgenic; Oligonucleotide Array Sequence Analysis; RNA, Messenger; RNA-Binding Proteins; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Ribonucleases; Spermatozoa; Testis; Transcription Factors; Transgenes
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