Extrachromosomal microDNAs and chromosomal microdeletions in normal tissues.
Science, 2012/4/06;336(6077):82-6.
Shibata Y[1], Kumar P, Layer R, Willcox S, Gagan JR, Griffith JD, Dutta A
Affiliations
PMID: 22403181DOI: 10.1126/science.1213307
Impact factor: 63.714
Abstract
We have identified tens of thousands of short extrachromosomal circular DNAs (microDNA) in mouse tissues as well as mouse and human cell lines. These microDNAs are 200 to 400 base pairs long, are derived from unique nonrepetitive sequence, and are enriched in the 5'-untranslated regions of genes, exons, and CpG islands. Chromosomal loci that are enriched sources of microDNA in the adult brain are somatically mosaic for microdeletions that appear to arise from the excision of microDNAs. Germline microdeletions identified by the "Thousand Genomes" project may also arise from the excision of microDNAs in the germline lineage. We have thus identified a previously unknown DNA entity in mammalian cells and provide evidence that their generation leaves behind deletions in different genomic loci.
MeSH terms
5' Untranslated Regions; Animals; Base Pairing; Base Sequence; Brain; Brain Chemistry; Cell Line; Cell Line, Tumor; Chromosome Deletion; Chromosomes, Human; Chromosomes, Mammalian; CpG Islands; DNA Replication; DNA, Circular; Exons; Germ Cells; Heart; Humans; Liver; Mice; Mice, Inbred C57BL; Microscopy, Electron; Molecular Sequence Data; Polymerase Chain Reaction; Repetitive Sequences, Nucleic Acid
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