Increased furan tolerance in Escherichia coli due to a cryptic ucpA gene.
Appl Environ Microbiol, 2012/4;78(7):2452-5.
Wang X[1], Miller EN, Yomano LP, Shanmugam KT, Ingram LO
Affiliations
PMID: 22267665DOI: 10.1128/AEM.07783-11
Impact factor: 5.005
Abstract
Expression arrays were used to identify 4 putative oxidoreductases that were upregulated (>3-fold) by furfural (15 mM, 15 min). Plasmid expression of one (ucpA) increased furan tolerance in ethanologenic strain LY180 and wild-type strain W. Deleting ucpA decreased furfural tolerance. Although the mechanism remains unknown, the cryptic ucpA gene is now associated with a phenotype: furan resistance.
MeSH terms
Alcohol Oxidoreductases; Culture Media; Drug Resistance, Bacterial; Escherichia coli; Escherichia coli Proteins; Ethanol; Fermentation; Furaldehyde; Furans; Gene Expression Regulation, Bacterial; Hydrogen-Ion Concentration; Molecular Sequence Data; NAD; Oligonucleotide Array Sequence Analysis; Up-Regulation; Xylose
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