Gene expression analysis of host innate immune responses in the central nervous system following lethal CVS-11 infection in mice.
Jpn J Infect Dis, 2011;64(6):463-72.
Sugiura N[1], Uda A, Inoue S, Kojima D, Hamamoto N, Kaku Y, Okutani A, Noguchi A, Park CH, Yamada A
Affiliations
PMID: 22116324
Impact factor: 2.541
Abstract
The central nervous system (CNS) tissue of mice infected with the CVS-11 strain of rabies virus (RABV) was subjected to gene expression analysis using microarray and canonical pathway analyses. Genes associated with innate immunity as well as inflammatory responses were significantly up-regulated, corroborating with the previous findings obtained using attenuated viruses that did not induce a fatal outcome in infected mice. Histopathological examination showed that neurons in the cerebellum had undergone apoptosis. Although the extent of Fas ligand up-regulation was not so prominent, perforin and granzyme genes were highly expressed in the CNS of mice infected with CVS-11. The presence of perforin and granzymes both in the Purkinje cells and CD3 T lymphocytes strongly suggested that apoptosis of the former cells was induced by the latter cells.
MeSH terms
Animals; Apoptosis; Cell Line; Central Nervous System; Cerebellum; Female; Gene Expression Profiling; Gene Expression Regulation; Histocytochemistry; Humans; Immunity, Innate; Mice; Mice, Inbred C57BL; Microarray Analysis; Neurons; Rabies; Rabies virus; Reverse Transcriptase Polymerase Chain Reaction
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