Embryonic stem cell-based mapping of developmental transcriptional programs.
Nat Methods, 2011/11/13;8(12):1056-8.
Mazzoni EO[1], Mahony S, Iacovino M, Morrison CA, Mountoufaris G, Closser M, Whyte WA, Young RA, Kyba M, Gifford DK, Wichterle H
Affiliations
PMID: 22081127DOI: 10.1038/nmeth.1775
Impact factor: 47.99
Abstract
The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.
MeSH terms
Animals; Cell Differentiation; Chromatin Immunoprecipitation; DNA-Binding Proteins; Embryonic Stem Cells; Gene Expression Regulation, Developmental; Genome-Wide Association Study; High-Throughput Nucleotide Sequencing; Mice; Motor Neurons; Sequence Analysis, DNA; Transcription Factors
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