Screening of freeze-dried protective agents for the formulation of biocontrol strains, Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40.
Lett Appl Microbiol, 2012/1;54(1):10-7.
Zhan Y[1], Xu Q, Yang MM, Yang HT, Liu HX, Wang YP, Guo JH
Affiliations
PMID: 22017666DOI: 10.1111/j.1472-765X.2011.03165.x
Impact factor: 2.813
Abstract
aims: The effects of different freeze-drying protective agents on the viabilities of biocontrol strains Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40 were investigated.
method and results: Several concentrations of protective and rehydration media were tested to improve the survival of biocontrol agents after freeze-drying. The subsequent survival rates during storage and rehydration media of freeze-dried biocontrol strains were also examined.
conclusions: The results indicated that cellobiose (5%) and d-galactose (5%) gave maximum viability of strains Bu. vietnamiensis B418 and P. agglomerans 2Re40 (98 and 54·3% respectively) while the perfect one (100%) of strain B. cereus AR156 was obtained with sucrose (5%) during freeze-drying, and the highest survival of the three strains was reached when they were rehydrated with 10% nonfat skim milk. In the following storage, the survival rates showed that B. cereus AR156 could still reach 50% after 12 months.
significance and impact of the study: This study showed that freeze-drying could be used to stabilize cells of these three biocontrol strains. Further studies should focus on the scale-up possibilities and formulation development.
MeSH terms
Animals; Bacillus cereus; Burkholderia; Cellobiose; Cryoprotective Agents; Galactose; Solanum lycopersicum; Microbial Viability; Milk; Pantoea; Pest Control, Biological; Plant Diseases; Sucrose
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