Microarray analysis shows that recessive resistance to Watermelon mosaic virus in melon is associated with the induction of defense response genes.
Mol Plant Microbe Interact, 2012/1;25(1):107-18.
Gonzalez-Ibeas D[1], Cañizares J, Aranda MA
Affiliations
PMID: 21970693DOI: 10.1094/MPMI-07-11-0193
Impact factor: 3.422
Abstract
Resistance to Watermelon mosaic virus (WMV) in melon (Cucumis melo L.) accession TGR-1551 is characterized by a significant reduction in virus titer, and is inherited as a recessive, loss-of-susceptibility allele. We measured virus RNA accumulation in TGR-1551 plants and a susceptible control ('Tendral') by real-time quantitative polymerase chain reaction, and also profiled the expression of 17,443 unigenes represented on a melon microarray over a 15-day time course. The virus accumulated to higher levels in cotyledons of the resistant variety up to 9 days postinoculation (dpi) but, thereafter, levels increased in the susceptible variety while those in the resistant variety declined. Microarray experiments looking at the early response to infection (1 and 3 dpi), as well as responses after 7 and 15 dpi, revealed more profound transcriptomic changes in resistant plants than susceptible ones. The gene expression profiles revealed deep and extensive transcriptome remodeling in TGR-1551 plants, often involving genes with pathogen response functions. Overall, our data suggested that resistance to WMV in TGR-1551 melon plants is associated with a defense response, which contrasts with the recessive nature of the resistance trait.
MeSH terms
Citrullus; Cluster Analysis; Cotyledon; Cucumis melo; Gene Expression Profiling; Genes, Plant; Genotype; Molecular Sequence Annotation; Oligonucleotide Array Sequence Analysis; Plant Diseases; Plant Immunity; Potyvirus; RNA, Plant; Seedlings; Time Factors; Transcriptional Activation; Transcriptome; Viral Load
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