The Vitis vinifera C-repeat binding protein 4 (VvCBF4) transcriptional factor enhances freezing tolerance in wine grape.
Plant Biotechnol J, 2012/1;10(1):105-24.
Tillett RL[1], Wheatley MD, Tattersall EA, Schlauch KA, Cramer GR, Cushman JC
Affiliations
PMID: 21914113DOI: 10.1111/j.1467-7652.2011.00648.x
Impact factor: 13.263
Abstract
Chilling and freezing can reduce significantly vine survival and fruit set in Vitis vinifera wine grape. To overcome such production losses, a recently identified grapevine C-repeat binding factor (CBF) gene, VvCBF4, was overexpressed in grape vine cv. 'Freedom' and found to improve freezing survival and reduced freezing-induced electrolyte leakage by up to 2 °C in non-cold-acclimated vines. In addition, overexpression of this transgene caused a reduced growth phenotype similar to that observed for CBF overexpression in Arabidopsis and other species. Both freezing tolerance and reduced growth phenotypes were manifested in a transgene dose-dependent manner. To understand the mechanistic basis of VvCBF4 transgene action, one transgenic line (9-12) was genotyped using microarray-based mRNA expression profiling. Forty-seven and 12 genes were identified in unstressed transgenic shoots with either a >1.5-fold increase or decrease in mRNA abundance, respectively. Comparison of mRNA changes with characterized CBF regulons in woody and herbaceous species revealed partial overlaps, suggesting that CBF-mediated cold acclimation responses are widely conserved. Putative VvCBF4-regulon targets included genes with functions in cell wall structure, lipid metabolism, epicuticular wax formation and stress-responses suggesting that the observed cold tolerance and dwarf phenotypes are the result of a complex network of diverse functional determinants.
MeSH terms
Adaptation, Physiological; Amino Acid Sequence; Electrophoresis, Gel, Two-Dimensional; Freezing; Gene Expression Regulation, Plant; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Plant Proteins; Plant Shoots; Principal Component Analysis; RNA, Messenger; Regulon; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, Protein; Transcription Factors; Vitis; Wine; Wood
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