Sequence analysis of the 2009 pandemic influenza A H1N1 virus haemagglutinin gene from 2009-2010 Brazilian clinical samples.
Mem Inst Oswaldo Cruz, 2011/8;106(5):613-6.
Ferreira JL[1], Borborema SE, Brígido LF, Oliveira MI, Paiva TM, Santos CL
Affiliations
PMID: 21894383
Impact factor: 2.747
Abstract
In this paper, we analysed the haemagglutinin (HA) gene identified by polymerase chain reaction from 90 influenza A H1N1 virus strains that circulated in Brazil from April 2009-June 2010. A World Health Organization sequencing protocol allowed us to identify amino acid mutations in the HA protein at positions S220T (71%), D239G/N/S (20%), Y247H (4.5%), E252K (3.3%), M274V (2.2%), Q310H (26.7%) and E391K (12%). A fatal outcome was associated with the D239G mutation (p < 0.0001). Brazilian HA genetic diversity, in comparison to a reference strain from California, highlights the role of influenza virus surveillance for study of viral evolution, in addition to monitoring the spread of the virus worldwide.
MeSH terms
Brazil; Genetic Variation; Hemagglutinin Glycoproteins, Influenza Virus; Humans; Influenza A Virus, H1N1 Subtype; Influenza, Human; Molecular Sequence Data; Mutation; Pandemics; Phylogeny; RNA, Viral; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis
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