The Bacillus subtilis GntR family repressor YtrA responds to cell wall antibiotics.
J Bacteriol, 2011/10;193(20):5793-801.
Salzberg LI[1], Luo Y, Hachmann AB, Mascher T, Helmann JD
Affiliations
PMID: 21856850DOI: 10.1128/JB.05862-11
Impact factor: 3.476
Abstract
The transglycosylation step of cell wall synthesis is a prime antibiotic target because it is essential and specific to bacteria. Two antibiotics, ramoplanin and moenomycin, target this step by binding to the substrate lipid II and the transglycosylase enzyme, respectively. Here, we compare the ramoplanin and moenomycin stimulons in the Gram-positive model organism Bacillus subtilis. Ramoplanin strongly induces the LiaRS two-component regulatory system, while moenomycin almost exclusively induces genes that are part of the regulon of the extracytoplasmic function (ECF) σ factor σ(M). Ramoplanin additionally induces the ytrABCDEF and ywoBCD operons, which are not part of a previously characterized antibiotic-responsive regulon. Cluster analysis reveals that these two operons are selectively induced by a subset of cell wall antibiotics that inhibit lipid II function or recycling. Repression of both operons requires YtrA, which recognizes an inverted repeat in front of its own operon and in front of ywoB. These results suggest that YtrA is an additional regulator of cell envelope stress responses.
MeSH terms
Anti-Bacterial Agents; Bacillus subtilis; Bacterial Proteins; Cell Wall; Depsipeptides; Gene Expression Regulation, Bacterial; Oligosaccharides; Operon; Regulon
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download