Single cell transcriptomics of neighboring hyphae of Aspergillus niger.
Genome Biol, 2011/8/04;12(8):R71.
de Bekker C[1], Bruning O, Jonker MJ, Breit TM, Wösten HA
Affiliations
PMID: 21816052DOI: 10.1186/gb-2011-12-8-r71
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Abstract
Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories.
MeSH terms
Aspergillus niger; Gene Expression Profiling; Gene Expression Regulation, Fungal; Genes, Fungal; Hyphae; Oligonucleotide Array Sequence Analysis; RNA, Fungal; Single-Cell Analysis
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