Expression of HOXB genes is significantly different in acute myeloid leukemia with a partial tandem duplication of MLL vs. a MLL translocation: a cross-laboratory study.
Cancer Genet, 2011/5;204(5):252-9.
Liu HC[1], Shih LY, May Chen MJ, Wang CC, Yeh TC, Lin TH, Chen CY, Lin CJ, Liang DC
Affiliations
PMID: 21665178DOI: 10.1016/j.cancergen.2011.02.003
Impact factor: 2.169
Abstract
In acute myeloid leukemia (AML), the mixed lineage leukemia (MLL) gene may be rearranged to generate a partial tandem duplication (PTD), or fused to partner genes through a chromosomal translocation (tMLL). In this study, we first explored the differentially expressed genes between MLL-PTD and tMLL using gene expression profiling of our cohort (15 MLL-PTD and 10 tMLL) and one published data set. The top 250 probes were chosen from each set, resulting in 29 common probes (21 unique genes) to both sets. The selected genes include four HOXB genes, HOXB2, B3, B5, and B6. The expression values of these HOXB genes significantly differ between MLL-PTD and tMLL cases. Clustering and classification analyses were thoroughly conducted to support our gene selection results. Second, as MLL-PTD, FLT3-ITD, and NPM1 mutations are identified in AML with normal karyotypes, we briefly studied their impact on the HOXB genes. Another contribution of this study is to demonstrate that using public data from other studies enriches samples for analysis and yields more conclusive results.
MeSH terms
Adult; Aged; Aged, 80 and over; Cluster Analysis; Female; Gene Duplication; Gene Expression Regulation, Neoplastic; Histone-Lysine N-Methyltransferase; Homeodomain Proteins; Humans; Leukemia, Myeloid, Acute; Male; Middle Aged; Myeloid-Lymphoid Leukemia Protein; Nucleophosmin; Transcription Factors; Translocation, Genetic
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