An early inflammatory gene profile in visceral adipose tissue in children.
Int J Pediatr Obes, 2011/6;6(2-2):e360-3.
Tam CS[1], Heilbronn LK, Henegar C, Wong M, Cowell CT, Cowley MJ, Kaplan W, Clément K, Baur LA
Affiliations
PMID: 21609243DOI: 10.3109/17477166.2011.575152
Abstract
The aim of this study was to characterize expression profiles of visceral and subcutaneous adipose tissue in children. Adipose tissue samples were collected from children having elective surgery (n = 71, [54 boys], 6.0 ± 4.3 years). Affymetrix microarrays (n = 20) were performed to characterize the functional profile and identify genes of interest in adipose tissue. Visceral adipose tissue had an overrepresentation of Gene Ontology themes related to immune and inflammatory responses and subcutaneous adipose tissue had an overrepresentation of themes related to adipocyte growth and development. Likewise, qPCR performed in the whole cohort showed a 30-fold increase in haptoglobin (P = 0.005), 7-fold increase in IL-10 (P < 0.001), 8-fold decrease in VEGF (P = 0.01) and a 28-fold decrease in TBOX15 (P < 0.001) in visceral compared to subcutaneous adipose tissue. The inflammatory pattern in visceral adipose tissue may represent an early stage of the adverse effects of this depot, and combined with chronic obesity, may contribute to increased metabolic and cardiovascular risk.
MeSH terms
Biopsy; Child; Female; Gene Expression Profiling; Gene Expression Regulation; Haptoglobins; Humans; Inflammation; Inflammation Mediators; Interleukin-10; Intra-Abdominal Fat; Male; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; Subcutaneous Fat; T-Box Domain Proteins; Vascular Endothelial Growth Factor A
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