A rapid and scalable system for studying gene function in mice using conditional RNA interference.
Cell, 2011/4/01;145(1):145-58.
Premsrirut PK[1], Dow LE, Kim SY, Camiolo M, Malone CD, Miething C, Scuoppo C, Zuber J, Dickins RA, Kogan SC, Shroyer KR, Sordella R, Hannon GJ, Lowe SW
Affiliations
PMID: 21458673DOI: 10.1016/j.cell.2011.03.012
Impact factor: 66.85
Abstract
RNA interference is a powerful tool for studying gene function, however, the reproducible generation of RNAi transgenic mice remains a significant limitation. By combining optimized fluorescence-coupled miR30-based shRNAs with high efficiency ES cell targeting, we developed a fast, scalable pipeline for the production of shRNA transgenic mice. Using this system, we generated eight tet-regulated shRNA transgenic lines targeting Firefly and Renilla luciferases, Oct4 and tumor suppressors p53, p16(INK4a), p19(ARF) and APC and demonstrate potent gene silencing and GFP-tracked knockdown in a broad range of tissues in vivo. Further, using an shRNA targeting APC, we illustrate how this approach can identify predicted phenotypes and also unknown functions for a well-studied gene. In addition, through regulated gene silencing we validate APC/Wnt and p19(ARF) as potential therapeutic targets in T cell acute lymphoblastic leukemia/lymphoma and lung adenocarcinoma, respectively. This system provides a cost-effective and scalable platform for the production of RNAi transgenic mice targeting any mammalian gene. PAPERCLIP:
MeSH terms
Adenocarcinoma; Animals; Embryonic Stem Cells; Gene Knockdown Techniques; Lung Neoplasms; Mice; Mice, Transgenic; MicroRNAs; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma; RNA Interference; RNA Processing, Post-Transcriptional; RNA, Small Interfering; Signal Transduction; Wnt Proteins
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