Complex genetic, photothermal, and photoacoustic analysis of nanoparticle-plant interactions.
Proc Natl Acad Sci U S A, 2011/1/18;108(3):1028-33.
Khodakovskaya MV[1], de Silva K, Nedosekin DA, Dervishi E, Biris AS, Shashkov EV, Galanzha EI, Zharov VP
Affiliations
PMID: 21189303DOI: 10.1073/pnas.1008856108
Impact factor: 12.779
Abstract
Understanding the nature of interactions between engineered nanomaterials and plants is crucial in comprehending the impact of nanotechnology on the environment and agriculture with a focus on toxicity concerns, plant disease treatment, and genetic engineering. To date, little progress has been made in studying nanoparticle-plant interactions at single nanoparticle and genetic levels. Here, we introduce an advanced platform integrating genetic, Raman, photothermal, and photoacoustic methods. Using this approach, we discovered that multiwall carbon nanotubes induce previously unknown changes in gene expression in tomato leaves and roots, particularly, up-regulation of the stress-related genes, including those induced by pathogens and the water-channel LeAqp2 gene. A nano-bubble amplified photothermal/photoacoustic imaging, spectroscopy, and burning technique demonstrated the detection of multiwall carbon nanotubes in roots, leaves, and fruits down to the single nanoparticle and cell level. Thus, our integrated platform allows the study of nanoparticles' impact on plants with higher sensitivity and specificity, compared to existing assays.
MeSH terms
Aquaporin 2; Gene Expression Regulation, Plant; Laser Scanning Cytometry; Lasers; Lycopersicon esculentum; Microarray Analysis; Nanotubes, Carbon; Plant Leaves; Plant Roots; Polymerase Chain Reaction; Spectrum Analysis
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