Production of serotonin by dual expression of tryptophan decarboxylase and tryptamine 5-hydroxylase in Escherichia coli.
Appl Microbiol Biotechnol, 2011/3;89(5):1387-94.
Park S[1], Kang K, Lee SW, Ahn MJ, Bae JM, Back K
Affiliations
PMID: 21080162DOI: 10.1007/s00253-010-2994-4
Impact factor: 5.56
Abstract
A plant-specific biogenic amine, serotonin, was produced by heterologous expression of two key biosynthetic genes, tryptophan decarboxylase (TDC) and tryptamine 5-hydroxylase (T5H), in Escherichia coli. The native T5H, a cytochrome P450 enzyme, was unable to be functionally expressed in E. coli. Through a series of N-terminal deletions or additions of tagging proteins, we generated a functional T5H enzyme construct (GST∆37T5H) in which glutathione S transferase (GST) was translationally fused with the N-terminal 37 amino acid deleted T5H. Dual expression of GST∆37T5H and TDC using a pCOLADuet-1 E. coli vector produced serotonin at concentrations of approximately 24 mg l⁻¹ in the culture medium and 4 mg l⁻¹ in the cells. An optimum temperature of approximately 20 °C was required to achieve peak serotonin production in E. coli because the low induction temperature gave rise to the highest soluble expression of GST∆37T5H.
MeSH terms
Aromatic-L-Amino-Acid Decarboxylases; Culture Media; Cytochrome P-450 Enzyme System; Escherichia coli; Gene Expression; Genetic Vectors; Mutant Proteins; Recombinant Fusion Proteins; Serotonin
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