Gene expression profiles in mouse liver after long-term low-dose-rate irradiation with gamma rays.
Radiat Res, 2010/11;174(5):611-7.
Uehara Y[1], Ito Y, Taki K, Nenoi M, Ichinohe K, Nakamura S, Tanaka S, Oghiso Y, Tanaka K, Matsumoto T, Paunesku T, Woloschak GE, Ono T
Affiliations
PMID: 20954861DOI: 10.1667/RR2195.1
Impact factor: 3.372
Abstract
Changes in gene expression profiles in mouse liver induced by long-term low-dose-rate γ irradiation were examined by microarray analysis. Three groups of male C57BL/6J mice were exposed to whole-body radiation at dose rates of 17-20 mGy/day, 0.86-1.0 mGy/day or 0.042-0.050 mGy/day for 401-485 days with cumulative doses of approximately 8 Gy, 0.4 Gy or 0.02 Gy, respectively. The gene expression levels in the livers of six animals from each exposure group were compared individually with that of pooled sham-irradiated animals. Some genes revealed a large variation in expression levels among individuals within each group, and the number of genes showing common changes in individuals from each group was limited: 20 and 11 genes showed more than 1.5-fold modulation with 17-20 mGy/day and 0.86-1.0 mGy/day, respectively. Three genes showed more than 1.5-fold modulation even at the lowest dose-rate of 0.04-0.05 mGy/day. Most of these genes were down-regulated. RT-PCR analysis confirmed the expression profiles of the majority of these genes. The results indicate that a few genes are modulated in response to very low-dose-rate irradiation. The functional analysis suggests that these genes may influence many processes, including obesity and tumorigenesis.
MeSH terms
Animals; Dose-Response Relationship, Radiation; Gamma Rays; Gene Expression Profiling; Liver; Male; Mice; Mice, Inbred C57BL; Occupational Exposure; Oligonucleotide Array Sequence Analysis; Reverse Transcriptase Polymerase Chain Reaction; Time Factors
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