Primers for mating-type diagnosis in Diaporthe and Phomopsis: their use in teleomorph induction in vitro and biological species definition.
Fungal Biol, 2010/2-2010/3;114(2-3):255-70.
Santos JM[1], Correia VG, Phillips AJ
Affiliations
PMID: 20943136DOI: 10.1016/j.funbio.2010.01.007
Impact factor: 2.91
Abstract
Sexual reproduction in ascomycete fungi is governed by the mating-type (MAT) locus. The MAT loci of Diaporthe and its Phomopsis anamorphs differ in only one gene: MAT1-1-1 in mating-type MAT1-1 and MAT1-2-1 in mating-type MAT1-2. In order to diagnose mating-types in Diaporthe and Phomopsis and evaluate their usefulness in teleomorph induction in vitro and biological species delimitation, we designed primers that amplify part of the MAT1-1-1 and MAT1-2-1 genes. MAT phylogenies were generated and compared with ITS and EF1-α phylograms. Species recognised in the EF1-α phylogeny corresponded directly with those determined in the MAT phylogenies. ITS was shown to be highly variable resulting in a large number of phylogenetic species that were discordant with MAT and EF1-α species. Mating experiments were conducted to evaluate the existence of reproductive barriers between some isolates, and their anamorphic morphologies were compared. The primers proved to be useful in the mating-type diagnosis of isolates, selection of compatible mating pairs, and in the assessment of biological species boundaries.
MeSH terms
Ascomycota; DNA Primers; DNA, Fungal; DNA, Ribosomal Spacer; Fungal Proteins; Genes, Mating Type, Fungal; Molecular Sequence Data; Peptide Elongation Factor 1; Phylogeny; Sequence Analysis, DNA; Species Specificity
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