Sucrose transporter NtSUT4 from tobacco BY-2 involved in plant cell shape during miniprotoplast culture.
J Plant Res, 2011/5;124(3):395-403.
Okubo-Kurihara E[1], Higaki T, Kurihara Y, Kutsuna N, Yamaguchi J, Hasezawa S
Affiliations
PMID: 20941637DOI: 10.1007/s10265-010-0377-7
Impact factor: 3
Abstract
Sucrose plays an important role in several cellular processes since it is a general source of metabolic energy, serves as a precursor for starch and cellulose synthesis, and is a metabolic starting point for carboxylate- and amino acid synthesis. While plant vacuole is the main cellular storage pool, where sucrose accumulates to high concentrations, only a small number of vacuolar sugar transporters have been identified and characterized to date. We initially identified a vacuolar sucrose transporter (NtSUT4) from tobacco BY-2 cells and established transgenic tobacco BY-2 cell lines that overexpress NtSUT4-GFP (BY-SUTG cells). Using a model system for synchronous cell elongation in miniprotoplasts (evacuolated cells) prepared from tobacco BY-2 cells, we found that NtSUT4-GFP overexpression inhibited cell growth towards the cell major axis. Moreover, under the same conditions, we found that the cell walls were well stained by calcofluor in BY-SUTG cells than in wild type BY-2 cells. These results suggest that NtSUT4 is involved in cell shape via sucrose homeostasis in plant cells.
MeSH terms
Cell Line; Cell Membrane; Cell Wall; Cellulose; Cloning, Molecular; Cytoplasm; Genetic Complementation Test; Homeostasis; Membrane Transport Proteins; Phylogeny; Plant Proteins; Plants, Genetically Modified; Protoplasts; Sequence Analysis, Protein; Sucrose; Nicotiana; Vacuoles
More resources
EndNote: Download