Comparative analysis of the early transcriptome of Brucella abortus--infected monocyte-derived macrophages from cattle naturally resistant or susceptible to brucellosis.
Res Vet Sci, 2011/8;91(1):40-51.
Rossetti CA[1], Galindo CL[2], Everts RE[3], Lewin HA[3], Garner HR[2], Adams LG[4]
Affiliations
PMID: 20932540DOI: 10.1016/j.rvsc.2010.09.002
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Abstract
Brucellosis is a worldwide zoonotic infectious disease that has a significant economic impact on animal production and human public health. We characterized the gene expression profile of B. abortus-infected monocyte-derived macrophages (MDMs) from naïve cattle naturally resistant (R) or susceptible (S) to brucellosis using a cDNA microarray technology. Our data indicate that (1) B. abortus induced a slightly increased genome activation in R MDMs and a down-regulated transcriptome in S MDMs, during the onset of infection, (2) R MDMs had the ability to mount a type 1 immune response against B. abortus infection which was impaired in S cells, and (3) the host cell activity was not altered after 12 h post-B. abortus infection in R MDMs while the cell cycle was largely arrested in infected S MDMs at 12 h p.i. These results contribute to an improved understanding of how host responses may be manipulated to prevent infection by brucellae.
MeSH terms
Animals; Brucella abortus; Brucellosis, Bovine; Cattle; Disease Susceptibility; Down-Regulation; Gene Expression Profiling; Immunity, Innate; Macrophages; Oligonucleotide Array Sequence Analysis
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