A role for pectin de-methylesterification in a developmentally regulated growth acceleration in dark-grown Arabidopsis hypocotyls.
New Phytol, 2010/11;188(3):726-39.
Pelletier S[1], Van Orden J, Wolf S, Vissenberg K, Delacourt J, Ndong YA, Pelloux J, Bischoff V, Urbain A, Mouille G, Lemonnier G, Renou JP, Höfte H
Affiliations
PMID: 20819179DOI: 10.1111/j.1469-8137.2010.03409.x
Impact factor: 10.323
Abstract
• We focused on a developmentally regulated growth acceleration in the dark-grown Arabidopsis hypocotyl to study the role of changes in cell wall metabolism in the control of cell elongation. • To this end, precise transcriptome analysis on dissected dark-grown hypocotyls, Fourier transform infrared (FT-IR) microspectroscopy and kinematic analysis were used. • Using a cellulose synthesis inhibitor, we showed that the growth acceleration marks a developmental transition during which growth becomes uncoupled from cellulose synthesis. We next investigated the cellular changes that take place during this transition. FT-IR microspectroscopy revealed significant changes in cell wall composition during, but not after, the growth acceleration. Transcriptome analysis suggested a role for cell wall remodeling, in particular pectin modification, in this growth acceleration. This was confirmed by the overexpression of a pectin methylesterase inhibitor, which caused a delay in the growth acceleration. • This study shows that the acceleration of cell elongation marks a developmental transition in dark-grown hypocotyl cells and supports a role for pectin de-methylesterification in the timing of this event.
MeSH terms
Arabidopsis; Carboxylic Ester Hydrolases; Cell Wall; Cellulose; Darkness; Esterification; Gene Expression; Gene Expression Profiling; Gene Expression Regulation, Developmental; Hypocotyl; Oligonucleotide Array Sequence Analysis; Pectins; Spectroscopy, Fourier Transform Infrared
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