Induction of interferon-stimulated genes by Simian virus 40 T antigens.
Virology, 2010/10/25;406(2):202-11.
Rathi AV[1], Cantalupo PG, Sarkar SN, Pipas JM
Affiliations
PMID: 20692676DOI: 10.1016/j.virol.2010.07.018
Impact factor: 3.513
Abstract
Simian virus 40 (SV40) large T antigen (TAg) is a multifunctional oncoprotein essential for productive viral infection and for cellular transformation. We have used microarray analysis to examine the global changes in cellular gene expression induced by wild-type T antigen (TAg(wt)) and TAg-mutants in mouse embryo fibroblasts (MEFs). The expression profile of approximately 800 cellular genes was altered by TAg(wt) and a truncated TAg (TAg(N136)), including many genes that influence cell cycle, DNA-replication, transcription, chromatin structure and DNA repair. Unexpectedly, we found a significant number of immune response genes upregulated by TAg(wt) including many interferon-stimulated genes (ISGs) such as ISG56, OAS, Rsad2, Ifi27 and Mx1. Additionally, we also observed activation of STAT1 by TAg(wt). Our genetic studies using several TAg-mutants reveal an unexplored function of TAg and indicate that the LXCXE motif and p53 binding are required for the upregulation of ISGs.
MeSH terms
Animals; Antigens, Viral, Tumor; Cells, Cultured; Fibroblasts; Gene Expression Regulation; Interferons; Mice; Polyomavirus Infections; Simian virus 40; Tumor Virus Infections
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download