The activation potential of MOF is constrained for dosage compensation.
Mol Cell, 2010/6/25;38(6):815-26.
Prestel M[1], Feller C, Straub T, Mitlöhner H, Becker PB
Affiliations
PMID: 20620953DOI: 10.1016/j.molcel.2010.05.022
Impact factor: 19.328
Abstract
The H4K16 acetyltransferase MOF plays a crucial role in dosage compensation in Drosophila but has additional, global functions. We compared the molecular context and effect of MOF in male and female flies, combining chromosome-wide mapping and transcriptome studies with analyses of defined reporter loci in transgenic flies. MOF distributes dynamically between two complexes, the dosage compensation complex and a complex containing MBD-R2, a global facilitator of transcription. These different targeting principles define the distribution of MOF between the X chromosome and autosomes and at transcription units with 5' or 3' enrichment. The male X chromosome differs from all other chromosomes in that H4K16 acetylation levels do not correlate with transcription output. The reconstitution of this phenomenon at a model locus revealed that the activation potential of MOF is constrained in male cells in the context of the DCC to arrive at the 2-fold activation of transcription characteristic of dosage compensation.
MeSH terms
Acetylation; Animals; Dosage Compensation, Genetic; Drosophila; Drosophila Proteins; Female; Gene Expression Profiling; Histone Acetyltransferases; Male; Nuclear Proteins; Sex Factors; Transcriptional Activation; X Chromosome
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