Towards an optimized culture medium for the generation of mouse induced pluripotent stem cells.
J Biol Chem, 2010/10/01;285(40):31066-72.
Chen J[1], Liu J, Han Q, Qin D, Xu J, Chen Y, Yang J, Song H, Yang D, Peng M, He W, Li R, Wang H, Gan Y, Ding K, Zeng L, Lai L, Esteban MA, Pei D
Affiliations
PMID: 20595395DOI: 10.1074/jbc.M110.139436
Impact factor: 5.486
Abstract
Generation of induced pluripotent stem cells from somatic cells using defined factors has potential relevant applications in regenerative medicine and biology. However, this promising technology remains inefficient and time consuming. We have devised a serum free culture medium termed iSF1 that facilitates the generation of mouse induced pluripotent stem cells. This optimization of the culture medium is sensitive to the presence of Myc in the reprogramming factors. Moreover, we could reprogram meningeal cells using only two factors Oct4/Klf4. Therefore, iSF1 represents a basal medium that may be used for mechanistic studies and testing new reprogramming approaches.
MeSH terms
Animals; Cell Culture Techniques; Cells, Cultured; Culture Media, Serum-Free; Induced Pluripotent Stem Cells; Kruppel-Like Factor 4; Mice; Mice, Transgenic
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