MVH in piRNA processing and gene silencing of retrotransposons.
Genes Dev, 2010/5;24(9):887-92.
Kuramochi-Miyagawa S[1], Watanabe T, Gotoh K, Takamatsu K, Chuma S, Kojima-Kita K, Shiromoto Y, Asada N, Toyoda A, Fujiyama A, Totoki Y, Shibata T, Kimura T, Nakatsuji N, Noce T, Sasaki H, Nakano T
Affiliations
PMID: 20439430DOI: 10.1101/gad.1902110
Impact factor: 12.89
Abstract
VASA is an evolutionarily conserved RNA helicase essential for germ cell development. The mouse PIWI family proteins MILI and MIWI2 are involved in production of Piwi-interacting RNAs (piRNAs) in fetal male germ cells through a ping-pong amplification cycle. Expression of retrotransposons is elevated in MILI- and MIWI2-deficient male germ cells due to defective de novo DNA methylation, which is presumably caused by impaired piRNA expression. Here, we report that essentially the same abnormalities are observed in MVH (mouse VASA homolog)-deficient mice. Comprehensive analysis of piRNAs in MVH-deficient fetal male germ cells showed that MVH plays crucial roles in the early phase of the ping-pong amplification cycle.
MeSH terms
Animals; Argonaute Proteins; DEAD-box RNA Helicases; DNA Methylation; Gene Expression Regulation; Gene Silencing; Genes, Intracisternal A-Particle; Long Interspersed Nucleotide Elements; Male; Mice; Mice, Knockout; Protein Transport; Proteins; RNA, Small Interfering; Spermatogenesis; Testis
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