Involvement of the mannose phosphotransferase system of Lactobacillus plantarum WCFS1 in peroxide stress tolerance.
Appl Environ Microbiol, 2010/6;76(11):3748-52.
Stevens MJ[1], Molenaar D, de Jong A, de Vos WM, Kleerebezem M
Affiliations
PMID: 20363783DOI: 10.1128/AEM.00073-10
Impact factor: 5.005
Abstract
A Lactobacillus plantarum strain with a deletion in the gene rpoN, encoding the alternative sigma factor 54 (sigma(54)), displayed a 100-fold-higher sensitivity to peroxide than its parental strain. This feature could be due to sigma(54)-dependent regulation of genes involved in the peroxide stress response. However, transcriptome analyses of the wild type and the mutant strain during peroxide exposure did not support such a role for sigma(54). Subsequent experiments revealed that the impaired expression of the mannose phosphotransferase system (PTS) operon in the rpoN mutant caused the observed increased peroxide sensitivity.
MeSH terms
Bacterial Proteins; Gene Deletion; Lactobacillus plantarum; Molecular Sequence Data; Operon; Oxidative Stress; Peroxides; Phosphotransferases (Alcohol Group Acceptor); RNA Polymerase Sigma 54; Stress, Physiological
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