Rapid detection of influenza A pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR.
J Clin Microbiol, 2010/5;48(5):1884-7.
Hindiyeh M[1], Ram D, Mandelboim M, Meningher T, Hirsh S, Robinov J, Levy V, Orzitzer S, Azar R, Grossman Z, Mendelson E
Affiliations
PMID: 20220153DOI: 10.1128/JCM.02540-09
Impact factor: 11.677
Abstract
The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.
MeSH terms
Amino Acid Substitution; Antiviral Agents; Drug Resistance, Viral; Humans; Influenza A Virus, H1N1 Subtype; Influenza, Human; Molecular Sequence Data; Mutation, Missense; Neuraminidase; Oseltamivir; RNA, Viral; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Time Factors; Viral Proteins; Virology
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