MRC1-dependent scaling of the budding yeast DNA replication timing program.
Genome Res, 2010/6;20(6):781-90.
Koren A[1], Soifer I, Barkai N
Affiliations
PMID: 20219942DOI: 10.1101/gr.102764.109
Impact factor: 9.438
Abstract
We describe the DNA replication timing programs of 14 yeast mutants with an extended S phase identified by a novel genome-wide screen. These mutants are associated with the DNA replication machinery, cell-cycle control, and dNTP synthesis and affect different parts of S phase. In 13 of the mutants, origin activation time scales with the duration of S phase. A limited number of origins become inactive in these strains, with inactive origins characterized by small replicons and distributed throughout S phase. In sharp contrast, cells deleted of MRC1, a gene implicated in replication fork stabilization and in the replication checkpoint pathway, maintained wild-type firing times despite over twofold lengthening of S phase. Numerous dormant origins were activated in this mutant. Our data suggest that most perturbations that lengthen S phase affect the entire program of replication timing, rather than a specific subset of origins, maintaining the relative order of origin firing time and delaying firing with relative proportions. Mrc1 emerges as a regulator of this robustness of the replication program.
MeSH terms
Cell Cycle Proteins; Cell Separation; DNA Replication Timing; DNA, Fungal; Epistasis, Genetic; Flow Cytometry; Mutation; Reverse Transcriptase Polymerase Chain Reaction; S Phase; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins
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