Role of the Sinorhizobium meliloti global regulator Hfq in gene regulation and symbiosis.
Mol Plant Microbe Interact, 2010/4;23(4):355-365.
Gao M[1], Barnett MJ[2], Long SR[2], Teplitski M[1]
Affiliations
PMID: 20192823DOI: 10.1094/MPMI-23-4-0355
Impact factor: 3.422
Abstract
The RNA-binding protein Hfq is a global regulator which controls diverse cellular processes in bacteria. To begin understanding the role of Hfq in the Sinorhizobium meliloti-Medicago truncatula nitrogen-fixing symbiosis, we defined free-living and symbiotic phenotypes of an hfq mutant. Over 500 transcripts were differentially accumulated in the hfq mutant of S. meliloti Rm1021 when grown in a shaking culture. Consistent with transcriptome-wide changes, the hfq mutant displayed dramatic alterations in metabolism of nitrogen-containing compounds, even though its carbon source utilization profiles were nearly identical to the wild type. The hfq mutant had reduced motility and was impaired for growth at alkaline pH. A deletion of hfq resulted in a reduced symbiotic efficiency, although the mutant was still able to initiate nodule development and differentiate into bacteroids.
MeSH terms
Gene Expression Regulation, Bacterial; Host Factor 1 Protein; Hydrogen-Ion Concentration; Medicago truncatula; Plant Root Nodulation; Plant Roots; Sinorhizobium meliloti; Stress, Physiological; Symbiosis
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